Chiralpak OT(+) 10µm 250x4.6mm HPLC Column

The Chiralpak OT(+) HPLC column 250x4.6mm, 10µm, is modified with poly(triphenylmethyl methacrylate) on a 10 µm silica support. In the case of Chiralpak OT(+), the synthetic methacrylate polymer selector is coated rather than immobilised; the phase is therefore based on neither amylose nor cellulose. The polymeric helical structure gives rise to a different chiral recognition environment to that found in polysaccharide phases. Chiralpak OT(+) is primarily used for chiral HPLC separations where polymethacrylate selectivity offers advantages and methanol is a suitable mobile phase. As the OT polymer selector is more sensitive to alcohols, Chiralpak OT(+) should be used in accordance with the recommended temperature and solvent conditions. Typical applications include the separation of aromatic racemates and other chiral compounds that do not dissolve sufficiently on coated amylose or cellulose phases.

Performance Characteristics and Advantages

• Amylose- and cellulose-based polysaccharide phases with high enantioselectivity
• Immobilized Chiralpak phases with very broad solvent compatibility
• Coated Chiralpak phases with proven selectivity and good loading capacity
• Suitable for use in normal-phase HPLC, reverse-phase HPLC, polar-organic HPLC, and SFC
• Wide selection of complementary selectors for efficient chiral screening
• Specialized phases for amino acids, peptides, chiral acids, and ligand exchange methods

Additional Notes

Chiralpak includes both amylose-based and cellulose-based polysaccharide phases. In the immobilized phases, the chiral selector is fixed to the silica support. This allows for the use of significantly more organic solvents than with coated phases, which is particularly helpful when samples have limited solubility. Coated Chiralpak phases are based on polysaccharide derivatives physically applied to silica. They have a long history of use and often offer very high selectivity and good loading capacity. The most important limitation is solvent compatibility: mobile phases or sample solutions that can dissolve the polysaccharide layer must be avoided. Immobilized and coated variants with similar modifications are not necessarily chromatographically interchangeable. Immobilization increases robustness and solvent flexibility but can alter the spatial accessibility of the selector. Therefore, methods should be reviewed when switching between coated and immobilized phases and reoptimized as needed.

Legal Notices

Chiralpak is a registered trademark of Daicel Corporation, Osaka, Japan.