Chiralpak OP(+) 10µm 250x4.6mm HPLC Column

The Chiralpak OP(+) HPLC column 250x4.6mm, 10µm, based on a synthetic polymethacrylate modification on a 10 µm silica support. The modification of Chiralpak OP(+) consists of poly(diphenyl-2-pyridylmethyl methacrylate), which is physically coated onto the silica surface to act as a chiral polymer selector. Chiralpak OP(+) is therefore not an amylose or cellulose phase, but a special chiral phase based on polymethacrylate. The coated design offers inherent selectivity, but requires careful selection of the mobile phase, as unsuitable solvents may adversely affect the applied polymer film. Chiralpak OP(+) is typically used in chiral HPLC under polar-organic or normal-phase-like conditions. Methanol, in particular, plays an important role in this phase. Applications include the separation of enantiomers of small, aromatic and hydrophobic compounds for which conventional amylose or cellulose phases do not provide sufficient resolution.

Performance Characteristics and Advantages

• Amylose- and cellulose-based polysaccharide phases with high enantioselectivity
• Immobilized Chiralpak phases with very broad solvent compatibility
• Coated Chiralpak phases with proven selectivity and good loading capacity
• Suitable for use in normal-phase HPLC, reverse-phase HPLC, polar-organic HPLC, and SFC
• Wide selection of complementary selectors for efficient chiral screening
• Specialized phases for amino acids, peptides, chiral acids, and ligand exchange methods

Additional Notes

Chiralpak includes both amylose-based and cellulose-based polysaccharide phases. In the immobilized phases, the chiral selector is fixed to the silica support. This allows for the use of significantly more organic solvents than with coated phases, which is particularly helpful when samples have limited solubility. Coated Chiralpak phases are based on polysaccharide derivatives physically applied to silica. They have a long history of use and often offer very high selectivity and good loading capacity. The most important limitation is solvent compatibility: mobile phases or sample solutions that can dissolve the polysaccharide layer must be avoided. Immobilized and coated variants with similar modifications are not necessarily chromatographically interchangeable. Immobilization increases robustness and solvent flexibility but can alter the spatial accessibility of the selector. Therefore, methods should be reviewed when switching between coated and immobilized phases and reoptimized as needed.

Legal Notices

Chiralpak is a registered trademark of Daicel Corporation, Osaka, Japan.