Test Conditions
Column: HALO 90 Å PCS C18, 2.7µm, 2.1x100mm
Part Number: 92812-617
Mobile Phase A: Water, 0.1% Formic acid
Mobile Phase B: Acetonitrile, 0.1% Formic acid
Isocratic: 25% B
Flow Rate: 0.4mL/min
Back Pressure: 267bar
Temperature: 35°C
Injection volume: 0.5µL (40µg)
Sample Solvent: 75/25 Wasser/Acetonitril
Detection: PDA, 254nm
Flow Cell: 1µL
Data Rate: 100Hz
Response Time: 0.025sec
LC System: Shimadzu Nexera X2