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SEC-GPC Standards
GPC/SEC standards are specifically designed to calibrate and validate your GPC/SEC analyses. The standards in our range are carefully characterised and certified to enable accurate determination of molecular weight distribution and other important parameters. GPC/SEC standards cover a wide range of materials, including polystyrene, polymethyl methacrylate, polyethylene oxide and many more. They are available in different molecular weight ranges and concentrations to cover a wide range of applications.
Technical Data
When is which SEC standard used?
The SEC standard should be selected to match the target analyste. The separation technique (aqueous or organic SEC) plays an important role here. The standards should also be selected so that they are as similar as possible to the analyte. Protein standards and dextran standards can serve as examples. Proteins tend to be globular and folded. This means that the proteins have a smaller hydrodynamic radius. Dextran standards are rather long chains and the hydrodynamic radius is therefore larger. As a result, proteins with the same pore size have a significantly larger molecular weight range compared to dextrans.
Polystyrene (PS)
- Use: In organic solvents (e.g. THF, chloroform).
- Use: Most common calibration standards in classical GPC.
- Typical samples: Polymers, resins, plastics.
- Advantage: Wide range of molecular weights, well characterised.
- Caution: Not ideal for branched polymers or biological samples.
Pullulan
- Application: In aqueous systems.
- Use: For water-soluble, mostly linear polysaccharides.
- Typical samples: Sugar, biopolymer products.
- Advantage: Monodisperse, very soluble.
- Disadvantage: Unlike proteins, folded - limited informative value for protein analyses.
Proteins (e.g. BSA, thyroglobulin, ovalbumin)
- Application: In biological or pharmaceutical SEC.
- Use: Calibration for proteins, antibodies, enzymes.
- Typical samples: Therapeutic proteins, mAbs, vaccines.
- Advantage: Physiologically relevant structure.
- Note: Hydrodynamic size (not MW) is decisive.
PEG/PEO (polyethylene glycol/oxide)
- Application: Aqueous or organic - depending on solubility.
- Use: For polymers, active pharmaceutical ingredients, biopolymers.
- Typical samples: PEGylated proteins, polymers.
- Advantage: Broad MW spectrum, good water solubility.
PMMA (polymethyl methacrylate)
- Application: In organic systems.
- Application: Alternative to polystyrene where density differs.
- Typical samples: Speciality polymers, technical polymers.
- Advantage: Comparison to materials with similar behaviour.
Dextran
- Application: In aqueous eluents (e.g. buffers, water).
- Use: Calibration standard for polysaccharides and biopolymers.
- Typical samples: Glycoproteins, polysaccharides, soluble dietary fibres.
- Advantage: Similar to pullulan in structure, but often available in a wider molecular range.
- Note: Slightly higher flexibility than pullulan - has an effect on hydrodynamic volume.
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